|
Immunochromatographic rapid test for the detection of Plasmodium falciparum in whole blood.
| - RELIABLE : |
|
- Quality system certified ISO 9001 and ISO 13485.
- Sensitivity : 100 %.
- Specificity : 97,7 %.
(An hospital evaluation was run comparatively to microscopic examination of thick (Giemsa) and thin (Diff-quick) blood smears as per WHO standards (40000 RBC for thin smear and 1000 WBC for thick smear)
|
|
| - PACKAGING AND PRESENTATION : |
|
- Kit of 25 tests containing :
>>>>>>>1 dropper vial containing the lysis buffer,
>>>>>>>25 dilution tubes,
>>>>>>>1 cardboard workstation.
- Storage : 2 - 30°C.
|
|
| - EFFICIENT : |
|
- Detection limit : 100 parasites/µl of blood and 10 parasites/µl of whole blood for the thick smear.
- FAST: Results at 5 minutes.
|
|
| - PRACTICAL : |
|
- SIMPLE: One step test.
- Do not require any instrumentation.
- Objective reading : presence or absence of color bands.
|
|
PRINCIPLE
IMMUNOQUICK Malaria falciparum is a simple and rapid test making it possible to detect the in vitro presence of Plasmodium falciparum in blood. The test uses a system of capture of the specific soluble antigen of Plasmodium falciparum : “Histidin rich protein II” (PfHRP – II) which is present and diffuse from infected red blood cells.
A monoclonal capture antibody is immobilized on the nitrocellulose membrane. The red blood cells are lysed by a lysis buffer thus releasing the antigen PfHRP II which will be migrating up to the capture antibody along the nitrocellulose membrane. The coloured signal is generated by black latex particles on which is immobilized a specific monoclonal antibody which will bind to the antibody-antigen complex thus leading to the appearance of a black band. The presence of a purple control band in the higher part of the test makes it possible to validate the correct procedure of the test.
|
